Source code for test_thermodynamics
# -*- coding: utf-8 -*-
# Copyright 2018 Novo Nordisk Foundation Center for Biosustainability,
# Technical University of Denmark.
#
# Licensed under the Apache License, Version 2.0 (the "License");
# you may not use this file except in compliance with the License.
# You may obtain a copy of the License at
#
# http://www.apache.org/licenses/LICENSE-2.0
#
# Unless required by applicable law or agreed to in writing, software
# distributed under the License is distributed on an "AS IS" BASIS,
# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
# See the License for the specific language governing permissions and
# limitations under the License.
"""Perform tests using eQuilibrator API on an instance of ``cobra.Model``."""
from __future__ import absolute_import, division
import pytest
pytest.skip(
"Thermodynamic tests are disabled until upgrade to new equilibrator-api version.",
allow_module_level=True,
)
import memote.support.basic as basic # noqa
import memote.support.thermodynamics as thermo # noqa
from memote.utils import annotate, get_ids, wrapper # noqa
@annotate(
title="Thermodynamic Reversibility of Purely Metabolic Reactions",
format_type="percent",
[docs])
def test_find_candidate_irreversible_reactions(model):
"""
Identify reversible reactions that could be irreversible.
If a reaction is neither a transport reaction, a biomass reaction nor a
boundary reaction, it is counted as a purely metabolic reaction.
This test checks if the reversibility attribute of each reaction
agrees with a thermodynamics-based
calculation of reversibility.
Implementation:
To determine reversibility we calculate
the reversibility index ln_gamma (natural logarithm of gamma) of each
reaction
using the eQuilibrator API. We consider reactions, whose reactants'
concentrations would need to change by more than three orders of
magnitude for the reaction flux to reverse direction, to be likely
candidates of irreversible reactions. This assume default concentrations
around 100 μM (~3 μM—3 mM) at pH = 7, I = 0.1 M and T = 298 K. The
corresponding reversibility index is approximately 7. For
further information on the thermodynamic and implementation details
please refer to
https://doi.org/10.1093/bioinformatics/bts317 and
https://pypi.org/project/equilibrator-api/.
Please note that currently eQuilibrator can only determine the
reversibility index for chemically and redox balanced reactions whose
metabolites can be mapped to KEGG compound identifiers (e.g. C00001). In
addition
to not being mappable to KEGG or the reaction not being balanced,
there is a possibility that the metabolite cannot be broken down into
chemical groups which is essential for the calculation of Gibbs energy
using group contributions. This test collects each erroneous reaction
and returns them as a tuple containing each list in the following order:
1. Reactions with reversibility index
2. Reactions with incomplete mapping to KEGG
3. Reactions with metabolites that are problematic during calculation
4. Chemically or redox unbalanced Reactions (after mapping to KEGG)
This test simply reports the number of reversible reactions that, according
to the reversibility index, are likely to be irreversible.
"""
# With gamma = 1000, ln_gamma ~ 6.9. We use 7 as the cut-off.
threshold = 7.0
ann = test_find_candidate_irreversible_reactions.annotation
met_rxns = basic.find_pure_metabolic_reactions(model)
(
rev_index,
incomplete,
problematic,
unbalanced,
) = thermo.find_thermodynamic_reversibility_index(met_rxns)
ann["data"] = (
# The reversibility index can be infinite so we convert it to a JSON
# compatible string.
[(r.id, str(i)) for r, i in rev_index],
get_ids(incomplete),
get_ids(problematic),
get_ids(unbalanced),
)
num_irrev = sum(1 for r, i in rev_index if abs(i) >= threshold)
ann["message"] = wrapper.fill(
"""Out of {} purely metabolic reactions, {} have an absolute
reversibility index greater or equal to 7 and are therefore likely
candidates for being irreversible.
{} reactions could not be mapped to KEGG completely, {} contained
'problematic' metabolites, and {} are chemically or redox imbalanced.
""".format(
len(met_rxns), num_irrev, len(incomplete), len(problematic), len(unbalanced)
)
)
ann["metric"] = num_irrev / len(rev_index)